Reporter
NG5
Part:BBa_K2623025:Design
Designed by: Yunyun Hu Group: iGEM18_XMU-China (2018-10-02)
Archaeal ribosomal protein protein L7Ae fused with SpyCatcher and mRFP1.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1168
Illegal NheI site found at 1191 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2325
Illegal AgeI site found at 2437 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Leader sequence is necessary for targeting our protein to the periplasmic membrane.
Source
Partly from E.coli's genomic sequence.