Reporter
NG5

Part:BBa_K2623025:Design

Designed by: Yunyun Hu   Group: iGEM18_XMU-China   (2018-10-02)


Archaeal ribosomal protein protein L7Ae fused with SpyCatcher and mRFP1.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1168
    Illegal NheI site found at 1191
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2325
    Illegal AgeI site found at 2437
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Leader sequence is necessary for targeting our protein to the periplasmic membrane.


Source

Partly from E.coli's genomic sequence.


References